Fig. 5.
Sox9 interacts with ZFTA-RELA and coregulates oncogenic programs in ZRFUS EPN. (A) Volcano plot depicting IP-MS data of Sox9 interactome in ZRFUS EPN. Fold change was calculated over control samples of nuclear lysates incubated with beads only without antibody. IP-MS experiments were performed in independent biological triplicates (P < 0.05 and fold change of >2). (B) Venn diagram showing the number of unique and overlapping Sox9 binding partners in 3xCr HGG, human HGG and ZRFUS EPN, and (C) GO terms associated with these interaction partners. NF-κB signaling unique to the ZRFUS EPN-specific Sox9 interactome is highlighted in red. (D) Quantification of Sox9 binding to ZFTA-RELA in ZRFUS EPN from IP-MS data. (E) Sox9 co-IP experiments with Rela proteins from ZRFUS EPN lysates. (F) Schematic of ChIP-Seq experiments from ZRFUS EPN cells and heatmap profiles demonstrating co-occupancy between Sox9, HA, Rela, and H3K27ac.