Fig. 1.

Sampling and methods used to characterize weekly cycles of neurodegeneration in B. schlosseri colonies. Schematic depicting the various methods employed over the course of the investigation including neural complex dissection, confocal staining, histology, TEM, behavioral experiments and the intervals over which neural complex sampling was conducted (over the weekly cycle and with age in adult individuals). Genetically identical subclones prepared via incision and separation were used in diverse experimental groups.