Fig. 3.

GMF and cofilin independently promote debranching. Debranching rates (± SE) mediated by 50 nM GMF and 100 nM cofilin individually and together were measured and analyzed as in SI Appendix, Figs. S1 and S2 in physiological buffer. Experiments were performed in the absence of Tpm. Lifetime distributions and fits are presented in SI Appendix, Fig. S2C, and censoring-corrected debranching rates are given in SI Appendix, Table S2.