Fig. 5.

Rescue of AOAA-inhibited CBS-H₂S activity by Ser in cellular context. A H₂S production in HEK293 cells in response to 1/3/10 mM Ser, 150 µM AOAA or their combination was assessed by live fluorescent imaging using AzMC H₂S-selective probe. B Quantification of the normalized cellular fluorescence shown in the representative pictures in panel A. C, D) Cell viability (C) and cell proliferation (D) of HEK293 cells after exposure to increasing concentrations of Ser (0.15–10 mM). E–J Cellular bioenergetics of HEK293 cells was assessed by Seahorse flux analyzer in the presence of increasing Ser concentrations 1 mM (E), 3 mM (F) and 10 mM (G). Basal respiration (H), maximal respiration (I) and ATP production (J) were calculated from the Seahorse flux data and compared. Data represents mean ± SEM values of at least five independent experiments