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. 2022 Jul 21;13:4039. doi: 10.1038/s41467-022-31728-z

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© The Author(s) 2022

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 7 — a Schematics of AAV1-CMV-Cre injection into unilateral LHb and Cre-dependent AAV2-hSyn-DIO-mCherry injection into the DRN of Tph2-tTA::TetO-eArchT-EYFP transgenic mice. b mCherry-expressing cells (red) were observed in the DRN. 5-HT neurons were labeled by eArchT-EYFP (green) (scale bar 200 μm). Enlarged pictures of mCherry (c), ArchT-EYFP (d), DAPI (e), and their merge (f) (scale bar 50 μm). Arrowheads indicate ArchT-EYFP-expressing cells (without fill) and ArchT-EYFP- mCherry+ cells (filled). g Ratio of mCherry+ cells that colocalized with ArchT-EYFP (yellow, 29 cells (14.9%)) and without ArchT-EYFP (red, 166 cells (85.1%)) in the DRN (n = 3 biologically independent animals). h Schematics of AAV1-Cre injection into unilateral LHb and Cre-dependent AAV2-EF1α-DIO-eNpHR3.0-EYFP into the DRN to observe projections to forebrain areas. i–k EYFP-expressing fibers (green) were observed in the medial part of the VTA (i scale bar 500 μm, j scale bar 50 μm), and bilateral LHb (k scale bar 200 μm). Blue: DAPI. Source data are provided as a Source Data file.