Fig. 2.

CarA and CarH homologues in Myxococcales.

A. Heat map of the sequence identities of myxobacterial CarH/A homologues relative to CarA_Mx, CarH_Mx and their DBDs or CBDs (see Table S1 for species abbreviations).

B. Left: Structure of the CarH_Tt protomer (PDB code: 5C8D) with its DBD (DNA recognition helix and wing in dark blue) and its CBD, with bound AdoCbl (sticks in magenta with upper axial Ado group in cyan). Right: Weblogos of myxobacterial CarH/A homologues alongside the corresponding subdomains in CarH_Tt. Dots indicate DNA contacts (blue), Wx₉EH motif (red), lower axial B₁₂‐binding motif (magenta), dimer contacts (green) and dimer‐dimer interface (black), based on the CarH_Tt and DBD‐CarA_Mx structures, with filled dots indicating residues tested by mutational analysis (Navarro‐Avilés et al., 2007; Jost et al., 2015b).

C. Maximum likelihood phylogeny (blue dots >80% bootstrap values) for myxobacterial CarH and CarA homologues.