FIGURE 3.

Fluorescence spectra of GPx1 ALA5 (a), ALA6 (b), and ALA7 (c) at 25°C in absence (^___) and in presence ( ‐ ‐ ‐ ) of 5M urea; at 53°C in absence (– – –) and in presence (– ∙ – ∙ –) of 5 M urea. The excitation wavelength was set at 295 nm (bandwidth 3 nm) and the emission spectra were recorded between 310 and 450 nm (bandwidth 3.5 nm)