FIGURE 4.

BG‐PN colocalization and total GLAST expression is decreased in 7 weeks nNOS^−/− cerebella compared with WT. (a) Representative confocal images of WT and nNOS^−/− cerebella expressing GLAST (green) and CalB (magenta) with nuclei stained with DAPI (blue). Scale bar represents 50 μm. Representative image of GLAST‐CalB colocalization analysis are depicted in the third panel, with white representing common GLAST+CalB overlay. (b) Violin plots represent the area of GLAST+CalB overlay in relation to the total area of CalB staining within a consistent ROI, represented as % area. N = 4 biological replicates per group; WT n = 12 ROIs, nNOS^−/− n = 10 ROIs. p < .0001. (c) Representative western blot of total GLAST protein expression for 7 weeks WT and nNOS^−/− cerebella and the housekeeping protein GAPDH, along with bar graph representing GLAST expression normalized to GAPDH. N = 3 biological replicates per group. p = .0053