FIGURE 10.

Ca²⁺/Na⁺ influx is downregulated by NO application in nNOS^−/− BG. (a–d) Images from a–d represent calcium (red) and sodium (green) responses at baseline and 3 min after d‐aspartate application (scale bar represents 100 μm). The representative time course of Ca²⁺/Na⁺ influx in response to application of d‐aspartate, denoted by the red arrow. The black line represents the Ca²⁺/Na⁺ influx of nNOS^−/− BGs without a pretreatment (CON), while the orange line represents either nNOS^−/− BGs pre‐treated with SNAP (a), SNAP+PKG_i (b), UCPH (c), or NCX_i (d). Black, dashed line represents baseline Ca²⁺/Na⁺ conductance. (e) Bar graph represents the area under the curve (AUC) for each pretreatment visualized in a–d after 5 min of d‐aspartate treatment. N = 3 experimental replicates. CON n = 23 cells, SNAP n = 20 cells, SNAP+PKG_i n = 26 cells, UCPH n = 25 cells, NCX_i n = 25 cells. F(4, 130) = 4.723; p = .0014