Figure 3.
CD spectra of single‐ and double‐stranded oligonucleotides with α‐d and β‐d configurations. All measurements were performed at a concentration of 5 μM+5 μM single strand in 100 mM NaCl, 10 mM MgCl2, and 10 mM Na‐cacodylate, pH 7.0. The cell path length of the cuvette for the CD measurements was 5 mm. For pyrene click adducts, a 2 μM+2 μM single strand concentration was used. Black and red curves show the CD spectra of the single strands. Blue curves show the calculated spectra (sum of the CD spectra of the single strands). Green curves show the experimentally determined spectra.