Fig. 7. Single intravitreal CA77 injection reproduces neuroprotection obtained with systemic CA77 administration.

a Spider graphs of the ratio of the thickness of the outer nuclear layer (ONL) and inner nuclear layer (INL) in retinas of rd10 mice 7 days after receiving at P18 a single intravitreal injection of CA77 (40 µM) or vehicle. n = 4 (vehicle) and 7 (CA treated), from 3 independent experiments. b, c Toluidine blue staining in plastic embedded sections from the same animals. Representative images (b) and quantification of the number of rows per column (left) and OS length (right) in the semithin sections (c) are shown. n = 4 animals. d, e Cone (green) and rod (magenta) staining in cryosections of rd10 mice treated as in a. Representative images (d) and quantification of rod (left) and cone (right) arrestin in outer segment (OS) (e) are shown. Nuclei are highlighted with DAPI. n = 4 (for rod arrestin) and 6 (for cone arrestin) mice per condition. f, g Representative image of the immunostaining for GFAP in the same retinas (f) and corresponding quantification of the GFAP projections (g). n = 4. h, i Electroretinographic responses of rd10 mice intravitreally injected with CA77 or vehicle. Wave amplitudes (h) and representative waves of electroretinograms (i) are shown. n = 6 veh and 7 CA77. Individual values and mean + s.e.m. are shown. ANOVA of repeated measures was applied to data in a. Two-sided unpaired t-test was applied in the rest of analysis shown. *p < 0.05, **p < 0.01 and ***p < 0.001. Source data and exact p values are provided as a Source Data file.