Fig. 8. N-CoR1 expression is reduced in retinitis pigmentosa.

a Protein levels of N-CoR1 in retinas from wild type (WT) and rd10 mice at the indicated postnatal (P) days. Data from²³. Values are expressed as Z score. n = 4 mice/genotype/time. b Immunoblot for N-CoR1 in retinas of 2 WT and 3 rd10 mice. Ponceau staining is shown as loading control. c Immunostaining for N-CoR1 in cyrosections of WT and rd10 mice retinas at p25. Nuclei are highlighted with DAPI. Merged (DAPI and N-CoR1) (top) and single (N-CoR1) (bottom) images. Bottom: Higher magnification of boxed regions from the inner nuclear layer (INL) (top) and in the ganglion cell layer (GCL) (bottom). d Ncor1 mRNA levels in WT and rd10 mice treated from P18 to P25 with daily i.p. injection of vehicle or CA77 (40 mg/kg bw). Values are expressed as folds WT vehicle. n = 4 (WT) and 7 (rd10) mice. e Heat map of CMA network genes expression in retinal organoids from healthy (Control) and retinitis pigmentosa patients (RP) bearing the PDE6B mutation. D: days of organoid differentiation. D90-D180: features of mid-state disease; D230: features of late-state disease. CMA index¹⁴ is shown at the bottom. Data from²⁴. f, g CMA activation index (f) and ratio of NCOR1 to RARA mRNA levels (g) in the same samples as in e. RNA was isolated from 3-5 organoids from two independent differentiations. h mRNA levels of NCOR1 (left), RARA (middle) and NCOR1 to RARA ratio in retinal organoids from Control and RP patients bearing mutations in RP2 at D180. Data from²⁵ n = 3 individuals per diagnosis (minima, maxima, center, bounds of box, whiskers and percentile, are detailed in the Source Data File). Individual values and mean + s.e.m. are shown. Two-way ANOVA was used in a, One-way ANOVA with Tukey’s multiple comparison post-hoc test in d and two-sided paired t test in h. *p < 0.05 and **p < 0.01. ns, not significant. Uncropped blots are in Supplementary Fig. 10. Source data and exact p values are provided as a Source Data file.