Figure 2.

YFP⁺ naïve‐like CD4⁺ T cells have a naïve‐like gene expression profile but exhibit Th1 polarization. (A) Heatmap of relative gene expression between YFP^– naïve (live CD3⁺ CD4⁺ CD62L⁺ CD44^–), YFP⁺ naïve‐like, YFP^– T_EM (live CD3⁺ CD4⁺ CD62L^– CD44⁺), and YFP⁺ T_EM cells (n = 3). Each row represents a gene and each column a cell sample. Gene expression is shown as z‐score and coloured according to the scale on the right. (B) Volcano plot showing differential gene expression between YFP⁺ and YFP^– naïve‐like CD4⁺ T cells (log2‐fold change vs. adjusted p‐value). Genes more highly expressed in YFP⁺ cells are in red and those more highly expressed in YFP^– cells are in blue (p _adj < 0.05, |log2FC|>1, n = 3). Selected differentially expressed genes are labeled. (C) Gene set enrichment analysis (GSEA) demonstrating enrichment of T_H1 genes within the genes more highly expressed in naïve‐like YFP⁺ CD4⁺ cells versus naïve‐like YFP^– CD4⁺ cells. (D) As B, except showing differential gene expression between YFP⁺ and YFP^– T_EM CD4⁺ T cells (n = 3).