Figure 4.

YFP⁺ naïve‐like CD4⁺ T cells resist polarization toward other CD4⁺ T‐cell lineages. (A) Representative flow plots from three independent experiments (each comprising three replicates) showing cytokine expression (top) or T‐bet and YFP expression (bottom) in YFP^– and YFP⁺ naïve‐like (CD62L⁺ CD44^–) CD4⁺ T cells after in vitro activation with anti‐CD3/CD28 and culture with IL‐2. (B) Percentage of cultured YFP^– and YFP⁺ naïve‐like (CD62L⁺ CD44^–) CD4⁺ T cells and T_EM (CD62L^–CD44⁺) producing IFN‐γ or IL‐17A after activation with anti‐CD3/CD28 and culture with IL‐2 (median with range shown, n = 9), *p < 0.05, ****p < 0.0001 (Two‐way ANOVA). (C) Representative flow plots from three independent experiments (each comprising three replicates) showing cytokine expression from in vitro cultured YFP^– and YFP⁺ T_EM after activation with anti‐CD3/CD28 and culture with IL‐2. (D) Representative flow plots from three independent experiments (each comprising two replicates) showing cytokine expression in naïve‐like (CD62L⁺ CD44^–) YFP^– and YFP⁺ CD4⁺ T cells after activation with anti‐CD3/CD28 and polarization in T_H0, T_H1, T_H2 T_H17 and iTreg conditions in vitro. (E) Proportion of naïve‐like (CD62L⁺ CD44^–) YFP^– and YFP⁺ CD4⁺ T cells producing cytokines after activation with anti‐CD3/CD28 and polarization in T_H1, T_H2 T_H17 and iTreg conditions in vitro (median with range shown, n = 6).