Skip to main content
. 2022 Jul 21;13(7):634. doi: 10.1038/s41419-022-05091-2

Fig. 4. Mitophagy increases reactive oxygen species (ROS) and hypoxia inducible factor 1α (HIF1α) to induce pulmonary artery smooth muscle cell (PASMC) proliferation.

Fig. 4

A Confocal images showing ROS production in PASMCs with or without NAC (10 mM), as indicated by DCFH-DA fluorescent signal. n = 24–28 cells per group, scale bar:100 μm. B Confocal images of DCFH-DA in Ad-sh-scramble and Ad-sh-FUNDC1 PASMCs under normoxia or hypoxia. n = 27–39 cells per group, scale bar:100 μm. C Western blot showing HIF1α levels in cells as in (A). n = 6 independent experiments per group. D PCNA protein level, E CCK8 assay, and F EDU assay in cells as in (A). n = 5 independent experiments per group for D, n = 6 independent experiments per group for E, and n = 4–7 independent experiments per group for F. Data are presented as mean ± SD. In A, C and E, p values were determined by one-way ANOVA followed by Bonferroni post hoc analysis. In B, D and F, p values were determined by Kruskal–Wallis test followed by Dunn’s post hoc analysis.