Figure 2.

Characterization of three types of primary human ALI culture

Human air-liquid interface (ALI) cultures generated from human bronchial epithelial cells (HBECs) in house (B-ALI) or obtained fully differentiated (MucilAir or SmallAir) were characterized. (A) The trans-epithelial electrical resistance (TEER) was measured for each ALI culture type across multiple founding cell donors. Each data point represents a biological replicate; the bar represents the median. ∗p = 0.0244 (B-ALI versus MucilAir) and ∗∗∗∗p < 0.0001 (MucilAir versus SmallAir) as determined using Mann-Whitney U test. (B) Immunohistochemistry of ALI culture cryosections using cell-type-specific antibodies to detect basal (cytokeratin 5), ciliated (β-tubulin), goblet (mucin 5AC), and club cells (CC10) shown in green. Images are representative; nuclei are counter-stained blue (DAPI); scale bars represent 100 μm.