Table 1.

CCRD matrix (coded and actual values) with the response of aflatoxins B1 and B2 and fumonisin B1 concentration

Assay	X1	X2	AFLAB2 (ng/g)	AFLAB1 (ng/g)	FB1 (µg/g)
1	− 1 (6)	− 1 (0.6)	0.40	0.01	1.53
2	+ 1 (18)	− 1 (0.6)	0.42	0.35	0.68
3	− 1 (6)	+ 1 (1.0)	0.38	< LOD	0.43
4	+ 1 (18)	+ 1 (1.0)	0.01	< LOD	0.82
5	− 1.41 (3.54)	0 (0.8)	0.01	0.01	0.50
6	+ 1.41 (26.46)	0 (0.8)	0.19	< LOD	1.75
7	0 (12)	− 1.41 (0.52)	0.08	< LOD	0.53
8	0 (12)	+ 1.41 (1.28)	0.01	0.01	0.21
9	0 (12)	0 (0.8)	3.78	10.28	0.71
10	0 (12)	0 (0.8)	3.98	12.87	0.88
11	0 (12)	0 (0.8)	3.80	10.01	0.79

X₁: Steeping time (h); X₂: Lactic acid concentration (%). LOD AFLA B1 0.04 ng/g