TABLE 2.

Treatments used to simulate conditions for adhesion during passage through the upper gastrointestinal tract

Treatment	Description
Effect of pH	No cell pretreatment; the adhesion experiments were performed in 0.1 M citrate-phosphate buffers at pH 2.0, 3.0, 4.0, 5.0, 6.0, 7.0, and 8.0
Effect of acid plus pepsin	No cell pretreatment; the adhesion experiments were performed in 0.2 M HCl-KCl buffer (pH 3.0) containing 30 U of pepsin A (EC 3.4.23.1; (Sigma Chemical Co.) ml−1
Effect of bile	No cell pretreatment; the adhesion experiments were performed in 0.1 M phosphate buffer (pH 7.0) containing 3.0 g of porcine bile (Sigma Chemical Co.) liter−1
Effect of pancreatin	No cell pretreatment; Hylon VII was pretreated for 6 h at 37°C with 0.01 g of porcine pancreatin (Sigma Chemical Co.) g−1; the starch was washed twice, and the adhesion experiments were performed in 0.1 M phosphate buffer (pH 7.0)