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. 2022 May 26;14(3):527–551. doi: 10.1016/j.jcmgh.2022.05.007

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© 2022 The Authors

This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).

PMC Copyright notice

HIRA is required for recruitment of H3.3, RNAP II, and H3.3S31ph to established cccDNA. (A–I) cccDNA-ChIP analysis at 9 dpi in CTL vs HIRA-depleted and transcomplemented conditions (WT or W799A D800A mutant constructs). cccDNA ChIP was performed with (B) antibodies against HIRA, (C) cellular RNAP II, (D) H3.3, and its (E) phosphorylated form H3.3S31ph, (F) H3pan, and (G) H3.1/2. (H and I) No antibody (NoAb) or anti-E2F antibody served as ChIP technical negative controls. Graphs represent the means ± SEM of at least 3 independent experiments.