Table 2.
List of Antibodies Used in this Study
| Target | Company | Dilution |
|---|---|---|
| HBc for PLA and ChIP | Invitrogen SC2362651, Waltham, MA | 4 μg |
| HBc for IF and FACS | Invitrogen MA1-7607, Waltham, MA | 1:500 |
| HIRA for PLA, ChIP, and WB | Abcam Ab20655, Cambridge UK | 2.5 μg/1:500 |
| H3.3 for ChIP | Abcam Ab62642, Cambridge UK | 5 μg |
| H3.3S31ph for ChIP | Abcam ab92628, Cambridge UK | 5 μg |
| RNA polymerase II for ChIP | Diagenode C15200004, Liège, Belgium | 1 μg |
| BrdU for ChIP | BD Pharmingen 555627, San Diego, CA | 5 μg |
| E2F for ChIP | Santa Cruz Biotechnology, Dallas, TX | 5 μg |
| β-actin for WB | Abcam Ab6276, Cambridge UK | 1:10,000 |
| H3pan for ChIP | Diagenode C15410324, Liège, Belgium | 2 μg |
| H2A for ChIP | Diagenode C15410166, Liège, Belgium | 2 μg |
| H2B for ChIP | Diagenode C15410157, Liège, Belgium | 1 μg |
| H4 for ChIP | Diagenode C15410156, Liège, Belgium | 2 μg |
| H3.1/3.2 for ChIP | Active motif AB_2793710, Carlsbad, CA | 4 μg |
| HBc for WB | Dako B0586, Santa Clara, CA | 1:500 |
| HBs for IF | Abcam ab8636, Cambridge UK | 1:100 |
| Goat anti-mouse IgG (H+L) secondary antibody, HRP | Invitrogen 62-6520 Waltham, MA | 1:20,000 |
| Goat anti-rabbit IgG (H+L) secondary antibody, HRP | Invitrogen 31460, Waltham, MA | 1:10,000 |
| PML for IF | Abcam ab179466, Cambridge UK | 1:500 |
| NSE4 for ChIP | Abcepta AP9909a, San Diego, CA | 4 μg |
FACS, Fluorescence-activated cell sorting; H+L, heavy and light chains of the IgG molecule; HBs, hepatitis B surface; HRP, horseradish peroxidase; IF, immunofluorescence; PML, promyelocytic leukemia; WB, western blotting.