REAGENT or RESOURCE	SOURCE	IDENTIFIER
Biological Samples
Pregnant Sprague-Dawley rats	Charles River Laboratory (Portage, MI)	RRID:RGD_10395233
Critical Commercial Assays
BluePlus Cig (2.4% nicotine)	blu.com	NA
10x Genomics Chromium Next GEM Single Cell 3ʹ Reagent Kits v3.1.	10x Genomics	1000128
10x Genomics Chromium Single Cell ATAC Reagent Kits v1.1	10x Genomics	1000164
KAPA library quantification	Roche	KK4873
Protector RNase inhibitor	Sigma Aldrich	03335402001
PBS	Gibco	10010-023
Nuclei PURE lysis buffer	Sigma Aldrich	L9286-180ML
Nuclei PURE sucrose cushion solution	Sigma Aldrich	S9058-120ML
Nuclei PURE storage buffer	Sigma Aldrich	S9183-30ML
Isoflurane	PubChem	3763
IBA1 antibody	Cell Signaling Technology	Cat# 17198S; RRID: AB_2820254
Secondary antibody	EMD Millipore	Cat# 401393; RRID: AB_437797
Deposited Data
All sequencing fastq files generated in this project	Gene Expression Omnibus	GEO:GSE185538
Software and Algorithms
CellRanger	10x Genomics	V3.1.0
CellRanger-atac	10x Genomics	V1.2.0
Seurat	satijalab.org/Seurat/	V3.0
Signac	satijalab.org/signac/	V1.4.0
rGADEM	Droit et al., 2021	V2.40.0
TFBSTools	Tan G, Lenhard B. 2016	V1.30.0
JASPAR database	jaspar.genereg.net	V2020
Slingshot	Street K et al., 2018	V1.2.0
ShinyGo	bioinformatics.sdstate.edu/go	V0.66
Fastqc	Andrew S, 2010	V0.11.4
Other
QuantStudio 7 Flex Real-Time PCR System	Applied Biosystems	Cat No.44-856-98
NextSeq 550 Sequencing System	Illumina	https://www.illumina.com/systems/sequencing-platforms/nextseq.html
HiSeq 4000 System	Illumina	https://www.illumina.com/systems/sequencing-platforms/hiseq.html