Figure 2.

Cis effect on Ucp1 mRNA expression in F1 offspring of B6 and 129

(A) SVF from iWAT of B6, 129, and F1 mice were immortalized and induced to differentiate into thermogenic adipocyte differentiation. The cells were stained with Oil Red O at day 7 of differentiation. Scale bar, 100μm.

(B) Ppag, Fabp4, Nfia and Ucp1 were quantified by RT-qPCR at the indicated time points. One-way ANOVA followed by Bonferroni’ post-hoc test. (mean ± SEM; n = 3 independent samples; ∗p < 0.05, ∗∗p < 0.01; N.S., not significant).

(C) Allelic imbalance of the mRNA as well as genomic DNA at the indicated locus were evaluated by qPCR analysis (mean ± SEM; n = 3 independent samples; ∗p < 0.05, ∗∗p < 0.01; N.S., not significant; significance was determined by two-tailed Student’s t test).

(D) A table showing the read count as well as allelic imbalance of all the six SNPs that lies within Ucp1 mRNA. Raw mapped counts of RNA-seq to reference allele (B6) and alternative allele (129) are shown. Imbalance means the rate of the alternative count. p values were calculated using the binomial test. See also Figure S1.