Figure 1.

IL-18Rα is expressed on BM ILC progenitors. (A) The expression of IL-18Rα on BM progenitors of wild-type C57BL/6J mice was analyzed by flow cytometry. CLP, Flt3⁺αLP, Flt3^-αLP, and ILC2 were defined as follows respectively: lin^-IL-7Rα⁺ckit^intSca1^intST2^-Flt3⁺α4β7^-, lin^-IL-7Rα⁺ckit^intSca1^intST2^-Flt3⁺α4β7⁺, lin^-IL-7Rα⁺ckit^intSca1^intST2^-Flt3^-α4β7⁺, lin^-IL-7Rα⁺Sca1⁺ST2⁺. (B) Percentage of IL-18Rα⁺ cells in the progenitors in panel (A, C) Mean fluorescence intensity of IL-18Rα in IL-18Rα⁺ cells from CLP, Flt3⁺ αLP, Flt3^- αLP, and ILC2 in panel (A)(D) Percentage of IL-18Rα⁺ lymphocytes in peripheral tissues. (E) Mean fluorescence intensity of IL-18Rα in lymphocytes in peripheral tissues. (F) t-SNE analysis of lin-IL-7Rα⁺ cells in the BM, and expression of IL-7Rα, IL-18Rα, Flt3, ckit, α4β7, and Thy1 in 6 clusters. (G) Representative FACS plot showing the expression of progenitor and ILC-related markers in Thy1⁺α4β7^-, Thy1⁺α4β7⁺, and Thy1^-α4β7⁺ in BM IL-7Rα⁺IL-18Rα⁺ cells. Data are representative of more than three independent experiments with three mice in each group in panels (B–E). The data are presented as mean ± SEM in panels (B–E). Data are representative of three mice in panel (G).