Figure 2.

IL18Ra⁺ BM progenitor cells contain multipotent ILCPs. (A–C) In this study, 200~500 cells from Thy1⁺α4β7^-, Thy1⁺α4β7⁺, and Thy1^-α4β7⁺ subsets from the BM IL-7Rα⁺IL-18Rα⁺ population were FACS-sorted and cultured on OP9 or OP9-DL1 stromal cells with 20 ng/ml SCF and 20 ng/ml IL-7 for 12–14 days. (A) Expansion fold of the three subsets after culture. (B) Representative FACS plot showing the generated ILCs after culture. (C) Percentage of NK/ILC1, ILC2, and ILC3 in CD45⁺ cells generated from Thy1⁺α4β7^-, Thy1⁺α4β7⁺, and Thy1^-α4β7⁺ subsets cultured on OP9 (left) or on OP9-DL1 (right). (D) In vitro differentiation of index-sorted single BM lin^-IL-18Rα⁺IL-7Rα⁺ cells on OP9-DL1 for 14–16 days. Sixty-four out of 570 clonal progenies cultured without IL-33 (indicated as “-”) and 34 out of 568 clonal progenies cultured with 20 ng/ml IL-33 (indicated as “IL-33”) were analyzed. Data are representative of three or more independent experiments with six mice in each group in panels (A, C) The data are presented as mean ± SEM in panels (A, C).