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. 2022 Jul 8;13:923424. doi: 10.3389/fimmu.2022.923424

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Copyright © 2022 Xie, Zhang, Dai, Yue, Li, Qiu, Lu and Xu

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Trajectory analysis of scRNA-seq IL-18Ra⁺ BM progenitor cells. (A) A single cell trajectory was constructed from the dataset in Figure 2D (Cluster 6 cells) using Monocle package. Five distinct states were identified along the trajectory. (B) Distribution of five clusters identified in Figure 2D at five states along the trajectory in panel (A, C) Heatmap shows the expression profiles of lymphoid progenitor-associated and ILC-associated genes in five states along the trajectory in panel (A, D) Expression changes of the indicated genes related to ILCP and committed or differentiated ILCs along pseudotime from state 3 to state 1 and state 5 in panel (A).