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Endogenous expression and localization of vasopressin receptors in HCT8 cells. (A) An equal amount of proteins (60 µg) of total homogenates from HCT8 cells were blotted and probed with the indicated antibodies. Immunodetection revealed that V1aR and V2R are expressed in this cell model. Mouse kidney homogenates were used as a positive control. (B) Representative confocal images of immunofluorescence studies show that HCT8 cells express V1aR and V2R endogenously. Bar = 10 µm.
