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. 2022 Jul 8;10:919438. doi: 10.3389/fcell.2022.919438

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Copyright © 2022 Centrone, D’Agostino, Ranieri, Mola, Faviana, Lippolis, Silvestris, Venneri, Di Mise, Valenti and Tamma.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Functional characterization of endogenous V2R receptor in HCT8 cells. cAMP levels were evaluated by FRET experiments in HCT8 cells transiently transfected with H96, as described in the Methods section. 5 min of stimulation with 100 nM dDAVP significantly reduced the netFRET signals consistent with a significant increase in the cAMP levels compared with untreated cells. Data are expressed as means ± S.E.M. and analyzed by one-way ANOVA followed by Dunnett’s Multiple Comparison test (*p < 0.05 vs. untreated).