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. 2022 Jul 8;10:919438. doi: 10.3389/fcell.2022.919438

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Copyright © 2022 Centrone, D’Agostino, Ranieri, Mola, Faviana, Lippolis, Silvestris, Venneri, Di Mise, Valenti and Tamma.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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AQP3 gene expression in HCT8 cells. Real-Time PCR was performed as described in the Methods. Treatment with dDAVP significantly increased AQP3 mRNA level compared with untreated cells. Cotreatment with dDAVP and SR49059 significantly increased AQP3 mRNA compared to untreated cells. By contrast, cotreatment with dDAVP and tolvaptan did not alter the AQP3 mRNA level. Data are shown as mean ± S.E.M. of 6 independent experiments and analyzed by one-way ANOVA followed by Dunnett’s Multiple Comparison test (*p < 0.05 and **p < 0.01 vs. untreated; ^# p < 0.05 vs. DMSO).