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. 2022 Jul 8;10:919438. doi: 10.3389/fcell.2022.919438

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Copyright © 2022 Centrone, D’Agostino, Ranieri, Mola, Faviana, Lippolis, Silvestris, Venneri, Di Mise, Valenti and Tamma.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Effect of Vasopressin receptors on cell growth. Cells were left under basal conditions or treated as described in the methods section, stained with 0.2% Coomassie Blue solution, and the total area of the growth cells was measured using Metamorph software. Compared to untreated cells, treatment with dDAVP significantly decreased the cell total growth area. A similar effect was observed when cells were treated with 100 nM dDAVP and 100 nM tolvaptan for 72 h. Incubation with 100 nM SR49059 abolished the effect of dDAVP on cell growth. Data are presented as means ± S.E.M. of 6 independent experiments and analyzed by one-way ANOVA followed by Dunnett’s Multiple Comparison test (****p < 0.0001 vs. untreated; ^#### p < 0.0001 vs. DMSO).