FIGURE 2.

Microbiota diversity analysis. (A) Color coded rarefaction curves. For each group, the average values of α-diversity indexes with 95% confidence intervals were reported at different sequencing depth. (B) Color-coded box and whisker plots showing the distribution of the considered α-diversity estimators among groups. The presence of statistically significant difference between group of data were also reported. (C) PCoA plot of bacterial α-diversity based on Bray-Curtis dissimilarity and Unweighted UniFrac distance according to considered groups of subjects. For each group, the 95% confidence interval has been drawn. Numbers between parentheses represents the percentage of the total variance explained by the principal coordinates. (D) Color-coded box and whisker plots showing the distribution of principal coordinates for the ERA group between different time points. (E,F) Differential abundance analysis of taxa at genus and species levels with a mean relative abundance ≥1% determined in at least one group. (G) Distribution of SCFAs levels among groups. The Mann-Whitney U test and was performed to determine statistically significant differences between the control group and the RA one at both considered time-points. The Wilcoxon Signed-Ranks test was performed to assess significant differences between the different time-point considered for the ERA group. The * and ** difference is statistically significant.