REAGENT or RESOURCE	SOURCE	IDENTIFIER
Antibodies
Anti-Mo Ly-6G (Gr-1) Monoclonal Antibody (RB6-8C5), PE-Cyanine5 (1:100)	eBioscience	48-5931-82
CD11b Monoclonal Antibody (M1/70), Super Bright 600 (1:100)	eBioscience	63-0112-82
Anti-Mo CD45.1, PE-Cyanine5.5 (1:100)	eBioscience	45-0453-82
Chemicals, peptides, and recombinant proteins
Recombinant mouse IL-3	PeproTech	213-13
Recombinant human IL-6	PeproTech	200-06
Recombinant mouse SCF	PeproTech	250-03
2-Mercaptoethanol (BME)	Macklin	M6230
5-Fluorouracil (5-FU)	Selleck	S1209
Geneticin sulfate (G418)	TargetMol	T6512
Lipofectamine™ 2000	Thermo Fisher Scientific	11668019
Opti-MEM	Gibco	31985062
RPMI-1640	Gibco	C11875500CP
DMEM (high glucose)	Gibco	C11995500BT
FBS	Gibco	10099141C
1 × PBS (pH7.4)	Solarbio	P1010
Penicillin-Streptomycin Liquid	Solarbio	P1400
BSA	Solarbio	A8020
HEPES	Solarbio	H8090
Sodium pyruvate	Sigma-Aldrich	P5280
Polybrene	Sigma-Aldrich	H9268
Ammonium chloride	Sinopharm	10009617
Potassium bicarbonate	Sinopharm	20030218
EDTA	Sinopharm	10009617
Anhydrous ethanol	Sinopharm	10009259
Human TruStain FcXTM	BioLegend	422302
Staining buffer	BD Biosciences	554657
IC Fixation Buffer	eBioscience	00-8222-49
Critical commercial assays
Lineage Cell Depletion Kit, mouse	Miltenyi Biotec	130-090-858
Experimental models: Cell liness
HEK293T cells	ATCC	CRL-3216
Experimental models: Organisms/Strains
B6.SJL (CD45.1) mouse	A gift from Dr. Fudi Wang	N/A
C57BL/6 mouse	Beijing Vital Laboratory Animal Technology	213
Recombinant DNA
MSCV-neo-MLL-AF9	A gift from Dr. Jianjun Chen	N/A
pCL-ECO	A gift from Dr. Jianjun Chen	N/A
Softwareand Algorithms
BD FACSDiva Software	BD Biosciences	https://www.bdbiosciences.com/zh-cn/products/software/instrument-software/bd-facsdiva-software
GraphPad Prism 8.0 software	GraphPad Software, Inc.	http://www.graphpad.com/scientificsoftware/prism/
Other
60 mm dish	NEST	705001
6-Well plate	NEST	703001
15 mL centrifuge tube	NEST	601052
50 mL centrifuge tube	NEST	602052
1 mL Syringe (with 27G 0.5 inch Needle)	BD Biosciences	309623
3 mL Syringe (with 25G 1 inch Needle)	BD Biosciences	309582
0.22 μm filter	Millipore	SLGPR33RB
40 μm cell strainer	BD Falcon	352340
MS Columns	Miltenyi Biotec	130-042-201
0.5 mL MiniCollect tube K3E K3EDTA	Greiner Bio-One	450530
Cell counting plate	Countstar	12-0005-50
Sterile scalpel blade	JZ Surgical Instruments	J0B080 23#
X-ray irradiator	Rad Source	RS2000Pro
Refrigerated centrifuge	Thermo Fisher Scientific	ST16R
Countstar Automated Cell Counter	Countstar	IC 1000
Mouse tail vein injection device	KEW Basis	KW-XXY
Hematology analyzer	URIT, China	URIT-2900Vet Plus
Flow cell sorter	BD Biosciences	BD FACSAria III
OctoMACS Separator	Miltenyi Biotec	130-042-109
MACS MultiStand	Miltenyi Biotec	130-042-303
Lamp	OPPLE	E27