Component | Amount |
---|---|
LR Clonase™ II enzyme mix | 2 μL |
entry clone (50–150 ng) | 1–7 μL |
destination vector (cSFB 150 ng/μL) | 1 μL |
TE buffer, pH 8.0 | Up to 10 μL |
Component | Amount |
---|---|
LR Clonase™ II enzyme mix | 2 μL |
entry clone (50–150 ng) | 1–7 μL |
destination vector (cSFB 150 ng/μL) | 1 μL |
TE buffer, pH 8.0 | Up to 10 μL |