Reagent | Final concentration | Amount |
---|---|---|
NP-40 | 0.5% | 20 mL |
1 M Tris.HCl, pH8.0 | 20 mM | 80 mL |
5 M NaCl | 100 mM | 80 mL |
0.5 M EDTA | 1 mM | 8 mL |
ddH2O | n/a | Up to 4 L |
Total | n/a | 4 L |
Storage: Store for up to 6 months at 25°C.
Reagent | Final concentration | Amount |
---|---|---|
NP-40 | 0.5% | 20 mL |
1 M Tris.HCl, pH8.0 | 20 mM | 80 mL |
5 M NaCl | 100 mM | 80 mL |
0.5 M EDTA | 1 mM | 8 mL |
ddH2O | n/a | Up to 4 L |
Total | n/a | 4 L |
Storage: Store for up to 6 months at 25°C.