Turbonuclease buffer

Reagent	Final concentration	Amount
1 M Tris.HCl, pH8.0	50 mM	0.5 mL
1 M MgCl2	1 mM	10 μL
ddH2O	n/a	Up to 10 mL
Total	n/a	10 mL

Storage: fresh prepared with addition of proteinase inhibitors.