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. 2022 Jul 8;13:910868. doi: 10.3389/fendo.2022.910868

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Copyright © 2022 Cheng, Yao, Zhou, Sun, Meng, Wang, Xing, Wan and Hua

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Effects of RAGE knockdown on METTL3 expression and m⁶A RNA methylation levels in NIT-1 cells. (A–C) Immunoblotting of RAGE and METTL3 protein expression in NIT-1 cells transfected with RAGE siRNA (si-RAGE) or a nonspecific control siRNA (si-NC) and treated with 1 mM of MG for 24 h. (D) m⁶A levels in total RNA from NIT-1 cells treated as described for (A–C). Results are presented as the means ± SD of n = 3–4 independent experiments. ^* p < 0.05.