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. 2022 Jul 8;13:926773. doi: 10.3389/fimmu.2022.926773

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Copyright © 2022 Mohtashami, Li, Lee and Zúñiga-Pflücker

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Thymic engraftment of aged mice is dependent on proT cell dosage. Conditioned aged B6 CD45.2 mice were i.v. injected with 1 x 10⁶ TDBM from CD45.2 GFP⁺ mice and an increasing number of proT cells, in vitro-generated from CD45.1 mice, ranging from 0.5, 1, 2 and 4 x10⁶. Thymuses were harvested on D14 and assessed for the presence of T cell markers using flow cytometry. Representative plots are shown in (A). CD44 vs. CD25 panels were gated on CD4^- CD8^- DN cells. Percentage of thymic cells derived from CD45.1⁺ proT cells are shown in graph in (B) (n=4; error bars depict SEM; *p<0.05, **p<0.01, ***p<0.001. analyzed by two-way ANOVA). ns, not significant.