Figure 3.

Quantification of the percentage of the core-fucosylated N-glycan structures. HPLC quantification of N-glycans derived from endogenous HEK293T (A) and HepG2 (B) glycoproteins. N-glycans decorating SEAP glycoprotein overexpressed by HEK293T (C) and HepG2 (D) cell lines. ∗p < 0.05; ∗∗p < 0.01; ∗∗∗p < 0.001; and ∗∗∗∗p < 0.0001 as determined using one-way ANOVA with the Tukey post hoc test. Data are presented as mean ± SD. Each sample was run in three technical replicates. HEK293T, human embryonic kidney 293T cell line; ns, not significant, SEAP, secreted alkaline phosphatase.