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O-glycosylation fingerprinting of HEK293T cells. MALDI-TOF mass spectra of permethylated mucin-type Bn-O-glycans secreted to the culture medium were permethylated and analyzed in a positive-ion mode. Structural assignments based on biosynthetic knowledge were prepared using the GlycoWorkBench tool (2.1; EuroCarbDB). HEK293T, human embryonic kidney 293T cell line.
