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. 2022 Jun 27;298(8):102206. doi: 10.1016/j.jbc.2022.102206

Figure 8.

Figure 8

Quantification of intracellular GDP-mannose and GDP-fucose concentration in cells fed with mannose. Intracellular concentrations of GDP-mannose (A) and GDP-fucose (B) in the wildtype and SLC35C1 knockout HEK293T cells cultured for 48 h in the absence and presence of 5 mM mannose. ∗p < 0.05; ∗∗p < 0.01 as determined using two-tailed unpaired t test with Welch’s correction. Data are presented as mean ± SD. Each sample was run in three biological replicates. Comparison of relative incorporation of the radioactive fucose derived from the de novo pathway in wildtype (C) and SLC35C1 knockout (D) HEK293T cells. HEK293T, human embryonic kidney 293T cell line; ISTD, internal standard (GDP-glucose); ns, not significant.