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. 2022 Jun 27;298(8):102206. doi: 10.1016/j.jbc.2022.102206

Figure 9.

Figure 9

Relative contribution of the de novo and salvage pathways in glycan fucosylation.A, incorporation of the radioactive fucose derived from the GDP-fucose produced in the salvage pathway into N-glycans measured for the wildtype (full bars) and SLC35C1 knockout (dashed bars) HEK293T cells cultured in the presence (black contours) and absence (red contours) of fetal bovine serum (FBS). B, incorporation of the radioactive fucose derived from the GDP-fucose produced in the de novo pathway into N-glycans measured for the wildtype (full bar) and SLC35C1 knockout (dashed bar) HEK293T cells. Radioactivity was normalized for the amount of the starting material (total protein). ∗p < 0.05; ∗∗∗p < 0.001 as determined using two-tailed unpaired t test with Welch’s correction. Data are presented as mean ± SD. Each sample was run in three biological replicates. HEK293T, human embryonic kidney 293T cell line; ns, not significant.