Table 1.
Primers used in this study
| No. | Primer name | Primer sequence 5ʹ-3ʹ | Product length (bp) |
|---|---|---|---|
| Primers used in RT–PCR analysis with total RNA as a template | |||
| 1 | F_C1KO | CTTCCCCAGCTTGCGCCTG | 133 |
| 2 | R_C1KO | GCGGCCCACATTGTAGAAGGC | 133 |
| 3 | F_C1/C2KO | GCTTCTCTACTACTGCTTCTC | 233 |
| 4 | R_C1/C2KO | TCAAGCGCCGTCGCCAGAGCT | 233 |
| Primers used in PCR analysis with genomic DNA as a template | |||
| 5 | F_C1KO | CTTCCCCAGCTTGCGCCTG | 236 |
| 6 | R_C1KO | TTGTTCTTCACTGCTTCAGTC | 236 |
| 7 | F_C1/C2KO | ATTTCCCCCTCTTCATGACG | 165 |
| 8 | R_C1/C2KO | TTCTCATCCTATGGTTCCCCA | 165 |
| Primer used in gene amplification and site-directed mutagenesis | |||
| 9 | F_HAC1 | AAAAAGTCGACATGGCATACCCATACGACGTACCAGACT ACGCAATGAATAGGGCCCCTCTGAAGCGGT | NA |
| 10 | R_HAC1 | AAAAAGTCGACTCACACCCCCATGGCGCTCTT | NA |
| 11 | F_C1_420_PAM | ACGTCGGTGTAGCCTTCTACA | NA |
| 12 | R_C1_420_PAM | ACTTGAGGCAGAGGTTATTG | NA |