FIG. 2.
ClustalW alignment of the CTnERL target site for integration of the ermF region and the CTnDOT junction sequences at the left (LJ) and right (RJ) junctions of the ermF region. An asterisk indicates 100% nucleotide identity. The TGA stop codon of orf2 is underlined, and this codon is the point of divergence between the CTnERL and CTnDOT sequences at the left end of the ermF region. Potential stem-loop structures downstream of orf2 in CTnDOT and CTnERL target sequences are indicated by boldface type, and the directions of the inverted repeats are indicated by arrows above the appropriate sequences. Sequences that are part of the ermF region and hence are not present in CTnERL are indicated by a gray background. The 89 nucleotides of CTnERL between the stop codon of orf2 and the left and right junctions of the ermF region are not present in CTnDOT.