TABLE 3.
Sites of integration for the ermF region in Bacteroides isolates
| Straina | Size of product (bp)
|
Element(s) presentc | ||
|---|---|---|---|---|
| ermF region left junction (primers 1 and 3)b | ermF region right junction (primers 4 and 2)b | No ermF region CTnERL product (primers 1 and 2)b | ||
| WH104 | 1,024 | 368 | 625 | CTnERL, CTnDOT |
| WH106 | 1,024 | 300 | 625 | CTnERL, CTnDOT |
| WH111 | 1,024 | 300 | 625 | CTnERL, CTnDOT |
| DH3925 | 1,024 | 368 | 625 | CTnERL, CTnDOT |
| DH3927 | 1,024 | 368 | 625 | CTnERL, CTnDOT |
| DH3941 | 900 | 368 | 625 | CTnERL, CTnDOT |
| DH4087 | 1,024 | 368 | 625 | CTnERL, CTnDOT |
| DH4096 | 1,024 | 368 | 625 | CTnERL, CTnDOT |
| DH4114 | 900 | 368 | CTnDOT | |
| DH4172 | 1,024 | 368 | 625 | CTnERL, CTnDOT |
| BF12256 | 1,024 | 368 | CTnDOT | |
| BFAK87 | 1,024 | 368 | 625 | CTnERL, CTnDOT |
| BFCEST | 1,024 | 368 | CTnDOT | |
| BFERL | 1,024 | 368 | 625 | CTnERL, CTnDOT |
| BTDOT | 1,024 | 368 | CTnDOT | |
| BF7397 | 1,024 | 368 | CTnDOT | |
| BF7639 | 1,024 | 368 | CTnDOT | |
| BF8223 | 1,024 | 368 | 625 | CTnERL, CTnDOT |
| BF8928 | 1,024 | 368 | 625 | CTnERL, CTnDOT |
| BT4107d | 1,024 | 368 | CTnDOT | |
| BT4104d | 625 | CTnERL | ||
a
All strains analyzed in PCR experiments were known to cross-hybridize to probes specific for tetQ, the joined left and right ends of CTnDOT, ermF, and tetX (40).
b
The primers utilized in these experiments are shown in Fig. 1.
c
It is not known whether any of the strains has two copies of either a CTnERL element or a CTnDOT element, although it is clear that many strains contain both CTnERL and CTnDOT.
d
Strains BT4107 and BT4104 were included as control strains for CTnDOT and CTnERL, respectively.