TABLE 4.
Mobilization of the ermF element mob region
| Donor strain(s) | Mobilization frequencya (transconjugant/recipient)
|
|
|---|---|---|
| Tc− | Tc+ | |
| BT4100(pGW39.1) | <10−9 | NAc |
| BT4100(pGW39.1, pTC-COW) | <10−9 | <10−9 |
| BT4104(pGW39.1) | <10−9 | 10−5 |
| BT4107(pGW39.1) | <10−9 | 10−5 |
| BT4104(pLYL11) | <10−9 | 10−4 |
| BT4107(pLYL7oriTRK2) | <10−9 | <10−9 |
| BT4104(pLYL05) | 10−6 | 10−4 |
| MCR(pGW39.1), HB101(R751)b | <10−9 | <10−9 |
| MCR(pLYL7oriTRK2), HB101(R75)b | <10−9 | <10−9 |
a
Frequencies were calculated from a typical mating experiment by determining the number of transconjugants recovered per recipient cell at the end of the experiment. Similar results were obtained in several independent experiments. Bacteroides donor strains were cultured in the presence of tetracycline (Tc+) or in the absence of tetracycline (Tc−).
b
The frequencies of plasmid mobilization between Bacteroides and E. coli are the frequencies for E. coli HB101 recipients except for experiments in which E. coli EM24NR was the recipient.
c
NA, experiment not appropriate since the donor strain was sensitive to tetracycline.