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. 2022 Jul 8;13:910526. doi: 10.3389/fimmu.2022.910526

Figure 2.

Figure 2

(A–H) (A) Immunohistochemistry analysis for F4/80 on liver specimens obtained from C57Bl6J WT mice and transgenic mice for SB3 (TG/SB3) fed on CDAA for 12 weeks. Magnification 10x, scale bar 200µm, magnification 40x, scale bar 50µm. (B) ImageJ software analysis performed to evaluate the amount of F4/80 positive area in C57Bl6J WT mice and transgenic mice for SB3 (TG/SB3) fed on CDAA for 12 weeks. *p < 0.05 versus WT mice. (C) Quantitative real time PCR analysis of SB3 in C57Bl6J WT mice fed on control (WT) or CDAA diet (WT+CDAA). *p < 0.05 versus WT mice. Quantitative real time PCR analysis of TNFα (D), IL-1β (E) and CCL2 (F) in C57Bl6J WT mice and transgenic mice for SB3 (TG/SB3) fed on control CSAA diet (WT and TG/SB3) or CDAA diet for 12 weeks (CDAA and TG/SB3+CDAA). ***p < 0.001 and *p < 0.05 versus WT mice fed on control CSAA diet (WT) or versus TG/SB3 mice fed on CSAA diet; #p < 0.05 versus C57Bl6J WT mice fed on CDAA diet (CDAA). (G) Serum levels of alanine amino transferase (ALT) analyzed as a parameter of parenchymal injury in C57Bl6J WT mice and transgenic mice for SB3 (TG/SB3) fed on control CSAA diet (WT and TG/SB3) or CDAA diet for 12 weeks (CDAA and TG/SB3+CDAA). **p < 0.01 versus WT mice fed on control CSAA diet (WT), ##p < 0.01 versus WT mice fed on CDAA diet, §§p < 0.01 versus TG/SB3 mice fed on CSAA diet. (H) Hematoxylin eosin staining and score of steatosis and lobular inflammation in WT e TG/SB3 mice fed on CDAA diet for 12 weeks to evaluate steatosis and inflammation. Magnification 20x, scale bar 100µm.