Fig. 5.

IC50 values for 4T1 (A) and SkBr3 (B) cells after 48 ​h and 72 ​h treatment. The in vitro cytotoxicity of 4T1 (C, E) and SkBr3 (D, F) cells which are illustrated after 48 ​h and 72 ​h of treatment with different concentrations (0, 3.125, 6.25, 12.5, 25, 50, 100 ​μM) of Epi, Epi-Nio, Epi-Nio-HA. Propidium Iodide vs Annexin V. QB1: early apoptotic cells, QB2: late apoptotic cells, QB3: viable cells, QB4: Necrotic cells, and total apoptosis for 4T1 (G, I) and SkBr3 (H, J) for 4T1 (G) and SkBr3 (H) cell lines (Control: Untreated sample). All data are presented as mean ​± ​SD (n ​= ​3 independent assays). (∗∗∗:p ​< ​0.001, ∗∗:p ​< ​0.01, ∗:p ​< ​0.05).