Table 2.

Design of experiments using the RSM method to optimize the niosomal formulation of Epirubicin.

Run	Levels of independent variables					Dependent variables
	Lipid (μmol)	Surfactant: Cholesterol (molar ratio)	Surfactant type	Cholesterol (mg)	Surfactant (mg)	Size (nm)	PDI	Entrapment Efficiency (EE) (%)
1	250	1	Span 60	48.33	53.83	157.4	0.197	77.96
2	200	1	Span 80	38.67	42.86	190.7	0.211	71.53
3	300	2	Span 80	38.28	86.15	271.2	0.335	82.1
4	200	1	Span 20	38.67	34.65	189.3	0.289	70.22
5	250	1	Span 80	48.33	53.58	175.9	0.208	78.24
6	250	2	Span 20	31.90	58.03	264.5	0.367	71.31
7	250	0.5	Span 80	64.76	35.36	267.5	0.289	74.53
8	250	0.5	Span 60	64.76	35.53	255.3	0.242	68.95
9	250	1	Span 80	48.33	53.58	186.3	0.229	75.41
10	300	0.5	Span 80	77.72	42.43	291.2	0.321	62.9
11	300	2	Span 20	38.28	69.64	282.9	0.321	75.36
12	300	1	Span 80	58.00	64.29	250.1	0.264	77
13	300	2	Span 60	38.28	86.55	284.1	0.334	80.35
14	200	1	Span 60	38.67	43.06	169.3	0.219	66.4
15	200	2	Span 80	25.52	57.43	228.3	0.259	71.53
16	200	0.5	Span 60	51.81	28.42	271.2	0.345	69.32
17	250	1	Span 20	48.33	43.31	205.8	0.189	69.59
18	250	1	Span 60	48.33	53.83	165.3	0.151	81.19
19	250	1	Span 20	48.33	43.31	201.4	0.229	67.18
20	300	1	Span 60	58.00	64.59	240.1	0.225	83.23
21	200	0.5	Span 20	51.81	22.87	288.9	0.315	62.82
22	250	2	Span 80	31.90	71.79	224.6	0.219	72.32
23	250	1	Span 80	48.33	53.58	198.3	0.258	73.27
24	300	0.5	Span 60	77.72	42.63	254.3	0.389	69.83
25	250	2	Span 60	31.90	72.13	223.7	0.185	73.89
26	250	0.5	Span 20	64.76	28.58	315.8	0.374	68.48
27	200	2	Span 20	25.52	46.43	227.1	0.23	72.03
28	250	1	Span 20	48.33	43.31	196.4	0.195	64.64
29	200	0.5	Span 80	51.81	28.29	301.2	0.328	72.32
30	250	1	Span 60	48.33	53.83	183.5	0.184	82.54
31	300	0.5	Span 20	77.72	34.30	326.1	0.365	67.32
32	200	2	Span 60	25.52	57.70	209.8	0.175	76.15
33	300	1	Span 20	58.00	51.97	231.7	0.286	71.85