Figure 5.

Inducible STAT5B-CA expression in Treg cells promotes metabolic adaptation to HFD. (A) Schematic view of the generation of tamoxifen-inducible, Treg cell-specific STAT5B-CA overexpression mice and dietary regimens to the mice. (B) Body weight of HFD-fed Foxp3^{eGFP-Cre-ERT2/Y} (n = 6) and Foxp3^{eGFP-Cre-ERT2/Y}Rosa26^Stat5b-CA/wt (n = 6) mice. (C–F) Weight of BAT (C), gonadal WAT (D), inguinal WAT (E), and quadricep muscle (F) of Foxp3^{eGFP-Cre-ERT2/Y} (n = 9) and Foxp3^{eGFP-Cre-ERT2/Y}Rosa26^Stat5b-CA/wt (n = 5) mice. (G) Insulin tolerance test of HFD-fed Foxp3^{eGFP-Cre-ERT2/Y} (n = 8) and Foxp3^{eGFP-Cre-ERT2/Y}Rosa26^Stat5b-CA/wt (n = 5). Area under curve (AUC) is shown to the right. (H) pS473-Akt and total-Akt immunoblotting of BAT protein lysates from Foxp3^{eGFP-Cre-ERT2/Y} and Foxp3^{eGFP-Cre-ERT2/Y}Rosa26^Stat5b-CA/wt mice treated with saline or insulin for 30 min. Data are presented as mean ± SEM. n.s., not significant; *, p < 0.05; **, p < 0.01 by unpaired student’s t-test, one-way or two-way ANOVA.