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. 2022 Jul 8;13:874863. doi: 10.3389/fimmu.2022.874863

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Copyright © 2022 Zhang, Salgado, Liu, Moazzami, Hogquist, Farrar and Ruan

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Thermogenesis and lipid metabolism in Foxp3^YFP-Cre/YRosa26^Stat5b-CA/wt mice. (A) BAT histology of HFD-fed Foxp3^YFP-Cre/Y and Foxp3^YFP-Cre/YRosa26^Stat5b-CA/wt mice. (B, C) Expression of thermogenic genes in BAT (B) and iWAT (C) of HFD-fed Foxp3^YFP-Cre/Y (n = 7) and Foxp3^YFP-Cre/YRosa26^Stat5b-CA/wt (n = 5) mice. (D) Gonadal WAT histology of HFD-fed Foxp3^YFP-Cre/Y and Foxp3^YFP-Cre/YRosa26^Stat5b-CA/wt mice. (E) Cd36 and (F) Lpl gene expression in WAT of HFD-fed Foxp3^YFP-Cre/Y (n = 7) and Foxp3^YFP-Cre/YRosa26^Stat5b-CA/wt (n = 5) mice. (G) Expression of the Cd36 gene in duodenum of HFD-fed Foxp3^YFP-Cre/Y (n = 7) and Foxp3^YFP-Cre/YRosa26^Stat5b-CA/wt (n = 5) mice. (H) Oral lipid tolerance test of HFD-fed Foxp3^YFP-Cre/Y (n = 9) and Foxp3^YFP-Cre/YRosa26^Stat5b-CA/wt (n = 6) mice. Serum triglyceride levels were determined. Area under curve (AUC) is shown to the right. Data are presented as mean ± SEM. *, p < 0.05; **, p < 0.01; ***, p < 0.001 by unpaired student’s t-test.