TABLE 3.
Variable amino acids in predicted open reading frames of either genomic or cDNA copies of Sig1 amplified from T. weissflogii clone Actin
| Template | Clone | Amino acids at the following positionsa:
|
|||||||||||||||||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| 9 | 10 | 22 | 43 | 52 | 60 | 63 | 66 | 67 | 68 | 95 | 100 | 119 | 131 | 137 | 138 | 139 | 144 | 148 | 159 | 168 | 169 | 171 | 177 | 181 | 182 | 185 | 186 | ||
| Genomic | Reference | R | N | P | D | S | T | F | M | S | D | G | L | S | N | F | N | A | H | E | H | L | A | L | Y | D | K | T | M |
| 1 | . | . | . | . | . | . | L | . | . | . | . | . | Y | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | |
| 2 | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | G | . | . | S | |
| 3 | . | . | . | . | P | . | L | . | . | . | . | . | Y | . | . | . | . | . | D | R | . | . | . | . | . | . | . | . | |
| 4 | . | . | . | . | . | . | . | . | . | . | V | P | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | |
| 5 | . | . | . | . | P | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | R | . | . | |
| 6 | K | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | |
| 7 | G | . | . | N | . | . | . | . | . | . | . | . | . | . | . | . | V | . | . | . | . | . | . | . | . | . | . | . | |
| 8 | . | D | . | . | . | . | . | . | . | . | . | . | . | . | Y | . | . | . | . | . | . | . | . | . | . | . | . | . | |
| 9 | . | . | . | . | P | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | R | . | . | |
| 10 | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | R | . | . | . | . | . | . | . | . | . | . | |
| cDNA | 1 | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . |
| 2 | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | P | . | . | . | . | . | . | . | |
| 3 | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | P | . | |
| 4 | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | D | . | . | . | . | . | . | . | . | . | . | . | . | |
| 5 | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | |
| 6 | . | . | . | . | . | . | . | . | . | . | . | . | Y | . | . | . | . | . | D | . | . | . | . | . | . | . | . | . | |
| 7 | . | . | . | . | . | . | . | V | . | . | . | . | . | H | . | . | . | . | . | . | . | . | S | . | . | . | . | . | |
| 8 | . | . | S | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | H | . | . | . | . | |
| 9 | . | . | . | . | . | I | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | |
| 10 | . | . | . | . | . | . | . | . | P | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | |
| 11 | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | T | . | . | . | . | . | . | |
| 12 | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | |
| 13 | . | . | . | . | . | . | . | . | . | G | . | . | Y | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | |
The positions are numbered relative to the first predicted amino acid of the open reading frame. Roman type indicates conservative changes, italic type indicates moderate changes, and underlining indicates radical changes. A dot indicates that the amino acid is identical to the amino acid in the reference clone.